Anti-angiogenic activities of chitooligosaccharides

Chitooligosaccharides (COS) was obtained from chitosan by depolymerization with enzyme and analyzed by HPLC and TOF-MS, and the results indicated that the polymerization degree of COS was 2–18. In order to explore the anti-angiogenic activities of COS, the effect of COS on chicken chorioallantoic membrane (CAM) angiogenesis and on proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVECs) induced by human hepatoma carcinoma cells (HCC) culture fluid was measured.